phloroglucinol and silver nitrate enhances axillary shoot proliferation in nodal explants of vitex negundo l. âan aromatic medicinal plant
نویسندگان
چکیده
a reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant vitex negundo through in vitro culture. of different concentrations of n6-benzyladenine (ba) evaluated as supplements to murahige and skoog (ms) medium, ba at 1mg/l was effective in enhancing bud break and shoot regeneration. response of explants was also influenced by explanting seasons. early bud break and enhancement of shoot regeneration could be achieved by supplementing phloroglucinol (pg) at 100mg/l and silver nitrate (20 mg/l) in addition to ba (1 mg/l). these compounds appear to have synergistic effects on enhancement of high frequency multiple shoot regeneration. the number of shoots obtained in the presence of agno3 and pg was significantly higher (15.12±0.33) than other media tested. the efficacy of culture establishment with high percentage of responding explants is depending on seasonal factors in addition to culture conditions. among the different concentrations of iba (0.1, 0.5 and 1.0 mg/l) tested, 0.5mg/l iba was more effective for inducing roots within two weeks of culture and 85% of the microshoots produced long healthy root system. ex vitro rooting of micropropagated shoots was achieved by treating the basal end of the microshoots in iba solution (4000 ppm) followed by planting in plastic pots filled with a mixture of soil, sand and vermicompost (6:2:1). rooted microshoots were successfully established in the field with 80 percent survival rate after three months.
منابع مشابه
Phloroglucinol and Silver Nitrate Enhances Axillary Shoot Proliferation in Nodal Explants of Vitex negundo L. âan Aromatic Medicinal Plant
A reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant Vitex negundo through in vitro culture. Of different concentrations of N6-benzyladenine (BA) evaluated as supplements to Murahige and Skoog (MS) medium, BA at 1mg/l was effective in enhancing bud break and shoot regeneration. Response of explants was al...
متن کاملHigh-Frequency in Vitro Direct Shoot Regeneration from Nodal Explants of Hyssop Plant (Hyssopus officinalis L.)
Considering great medicinal value of Hyssopus officinalis L. and possibility of its mass production through in vitro culture, two individual experiments was conducted. Effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of TDZ and BAP in combination with 1 µmol of IAA on direct regeneration from nodal explants were assessed. Significant difference between treatments was observed (P≤ 0.01...
متن کاملPTC & B In vitro Regeneration of Vitex negundo L . - A Multi - purpose Woody Aromatic Medicinal Shrub
A rapid and efficient protocol was developed for inducing indirect organogenesis using leaf explants of Vitex negundo L. Explants were cultured on MS with different concentrations of 2,4-D and IAA in combination with BAP for callus induction. The frequency of callus induction increased with increasing concentration of IAA (0.3 mg/l) and BAP (0.3 mg/l) at optimal level. The shoot buds appeared e...
متن کاملDirect Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Solanum Nigrum L. A Medicinal Herb
In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0-5.0 mg/l) and KIN (1.0-5.0 mg/l) individually. Among various concentrations of cytokinins tested, maximum number of multiple sho...
متن کاملAn Efficient Multiple Shoot Induction Protocol from Nodal and Root Explants of Atalantia Monophylla (l.) Dc., a Medicinal Plant
Atalantia monophylla (L.) DC. is a medium sized medicinal tree used in Ayurveda, Siddha and Traditional systems of medicine. It is used for the treatment of chronic rheumatism and paralysis, as an antidote for snake bites and shows antibacterial, antifungal and antifeedant activity. The study aims to develop a protocol for multiple shoot induction from nodal and root explants. After surface ste...
متن کاملA Review on Vitex negundo L. – A Medicinally Important Plant
Vitex negundo L. (Verbenaceae) is a hardy plant, flourishing mainly in the Indian subcontinent. All parts of the plant, from root to fruit, possess a multitude of phytochemical secondary metabolites which impart an unprecedented variety of medicinal uses to the plant. It is interesting to note that a single plant species finds use for treatment of a wide spectrum of health disorders in traditio...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of biotechnologyناشر: national institute of genetic engineering and biotechnology
ISSN 1728-3043
دوره 8
شماره 2 2010
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023